SOD1 Antikörper (C-Term) (FITC)

Produktnummer ABIN2486226

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SOD1 in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SOD1 Antikörper (C-Term) (FITC) (ABIN2486226)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-SOD1 Antikörper (FITC)

Bindungsspezifität: C-Term

Spezifität: Recognizes a conformation specific epitope where the dimer interface is exposed.

Kreuzreaktivität: Human, Maus, Schwein, Ratte

Aufreinigung: Protein A Purified

Immunogen: C-terminal region of SOD1, exposed dimer interface (EDI)

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1μg/ml of ABIN2486226 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Andere Bezeichnung: SOD1

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1.

Gen-ID: 6647

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis