LAMP1 Antikörper (Atto 390)

Produktnummer ABIN2484737

Dieses Anti-LAMP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von LAMP1 in WB, IF, IP und ICC. Geeignet für Ratte.

Kurzübersicht für LAMP1 Antikörper (Atto 390) (ABIN2484737)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist konjugiert mit Atto 390

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: Ly1C6

Produktdetails anti-LAMP1 Antikörper (Atto 390)

Spezifität: Detects ~120 kDa.

Kreuzreaktivität: Hamster, Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Rat liver lysosomal membrane preparations

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: LAMP1

Hintergrund: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

Gen-ID: 25328

NCBI Accession: NP_036989

UniProt: P14562

Pathways: Autophagie