HDEL Antikörper (C-Term) (HRP)

Produktnummer ABIN2484715

Produktdetails anti-HDEL Antikörper (HRP)

Target: HDEL

Bindungsspezifität: C-Term

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HDEL Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~78 kDa.

Kreuzreaktivität: Drosophila melanogaster, Pflanzen, Saccharomyces cerevisiae

Aufreinigung: Protein G Purified

Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Klon: 2E7

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484715 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HDEL

Target: HDEL

Andere Bezeichnung: HDEL (HDEL Produkte)

Hintergrund: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).