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HDEL Antikörper (C-Term) (FITC)

HDEL Reaktivität: Saccharomyces cerevisiae WB, IF, ICC Wirt: Maus Monoclonal 2E7 FITC
Produktnummer ABIN2484714
  • Target Alle HDEL Produkte
    HDEL
    Bindungsspezifität
    C-Term
    Reaktivität
    • 22
    • 12
    • 11
    • 10
    • 10
    • 10
    • 10
    • 10
    • 1
    Saccharomyces cerevisiae
    Wirt
    • 22
    Maus
    Klonalität
    • 22
    Monoklonal
    Konjugat
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser HDEL Antikörper ist konjugiert mit FITC
    Applikation
    • 22
    • 12
    • 10
    • 10
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    Detects ~78 kDa.
    Kreuzreaktivität
    Drosophila melanogaster, Pflanzen, Saccharomyces cerevisiae
    Aufreinigung
    Protein G Purified
    Immunogen
    Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
    Klon
    2E7
    Isotyp
    IgG2b
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN2484714 was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    HDEL
    Andere Bezeichnung
    HDEL (HDEL Produkte)
    Hintergrund
    HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
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