Notch1 Antikörper (AA 20-216) (PerCP)

Produktnummer ABIN2483203

Der Maus Monoklonal Anti-Notch1-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, Notch1 in Proben von Maus zu detektieren.

Kurzübersicht für Notch1 Antikörper (AA 20-216) (PerCP) (ABIN2483203)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist konjugiert mit PerCP

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: S253-32

Produktdetails anti-Notch1 Antikörper (PerCP)

Bindungsspezifität: AA 20-216

Spezifität: Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2483203 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch1

Hintergrund: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Gen-ID: 18128

NCBI Accession: NP_032740

UniProt: Q01705

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development