DNAJB1 Antikörper (FITC)

Produktnummer ABIN2481541

Dieses Maus Monoklonal-Antikörper erkennt spezifisch DNAJB1 in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Saccharomyces cerevisiae.

Kurzübersicht für DNAJB1 Antikörper (FITC) (ABIN2481541)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNAJB1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 1G10-H8

Produktdetails anti-DNAJB1 Antikörper (FITC)

Spezifität: Detects ~40 kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

Kreuzreaktivität: Saccharomyces cerevisiae

Aufreinigung: Protein G Purified

Immunogen: Full length protein yeast HSP40 (YDJ1)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN2481541 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Andere Bezeichnung: HSP40

Hintergrund: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Gen-ID: 855661

NCBI Accession: NP_014335

UniProt: P25491