RAD52 antibody can be used in Western Blot at 1:1000, immunohistochemistry starting at 1:25 - 1:100, and immunoprecipitation starting at 1:50.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
As with all antibodies avoid freeze/thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
Store RAD52 antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 °C or below.
Target
RAD52
(RAD52 Homolog (S. Cerevisiae) (RAD52))
Andere Bezeichnung
RAD52
Hintergrund
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).