DARC Antikörper (N-Term)

Produktnummer ABIN2465620

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch DARC in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für DARC Antikörper (N-Term) (ABIN2465620)

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-DARC Antikörper

Bindungsspezifität: N-Term

Aufreinigung: Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.

Anwendungsinformationen

Applikationshinweise: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 μg/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Gen-ID: 2532

NCBI Accession: NP_002027

UniProt: Q16570