BTK Antikörper (truncated)

Produktnummer ABIN2464023

Produktdetails anti-BTK Antikörper

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Bindungsspezifität: truncated

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BTK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Immunogen: Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).

Klon: 7F12H4

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Lagerung: -20 °C

Informationen zur Lagerung: BTK monoclonal antibody can be stored at -20 °C, stable for one year.

Details zu BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Andere Bezeichnung: BTK (BTK Produkte)

Hintergrund: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Gen-ID: 695

UniProt: Q06187

Pathways: Fc-epsilon Rezeptor Signalübertragung, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling