TBP Antikörper

Produktnummer ABIN2463874

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TBP in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Zebrafisch (Danio rerio) und Hund.

Kurzübersicht für TBP Antikörper (ABIN2463874)

Target: TBP (TATA Box Binding Protein (TBP))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TBP Antikörper

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBP.

Anwendungsinformationen

Applikationshinweise: TBP antibody can be used for detection of TBP by ELISA at 1:62500. TBP antibody can be used for detection of TBP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TBP antibody at -20 °C.

Details zu TBP

Target: TBP (TATA Box Binding Protein (TBP))

Andere Bezeichnung: TBP

Hintergrund: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Molekulargewicht: 38 kDa

Gen-ID: 6908

NCBI Accession: NP_003185

UniProt: P20226

Pathways: WNT Signalweg