RFC3 Antikörper

Produktnummer ABIN2462934

Der Kaninchen Polyklonal anti-RFC3 Antikörper (ABIN2462934) detektiert spezifisch RFC3 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für RFC3 Antikörper (ABIN2462934)

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-RFC3 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC3.

Anwendungsinformationen

Applikationshinweise: RFC3 antibody can be used for detection of RFC3 by ELISA at 1:62500. RFC3 antibody can be used for detection of RFC3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC3 antibody at -20 °C.

Details zu RFC3

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Andere Bezeichnung: RFC3

Hintergrund: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molekulargewicht: 40 kDa

Gen-ID: 5983

NCBI Accession: NP_002906

UniProt: P40938

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA