NONO Antikörper

Produktnummer ABIN2462238

Produktdetails anti-NONO Antikörper

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NONO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NONO.

Anwendungsinformationen

Applikationshinweise: NONO antibody can be used for detection of NONO by ELISA at 1:312500. NONO antibody can be used for detection of NONO by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NONO antibody at -20 °C.

Details zu NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Andere Bezeichnung: NONO (NONO Produkte)

Synonyme: NMT55 antikoerper, NRB54 antikoerper, P54 antikoerper, P54NRB antikoerper, nmt55 antikoerper, nrb54 antikoerper, p54nrb antikoerper, xp54nrb antikoerper, AA407051 antikoerper, AV149256 antikoerper, nonA antikoerper, non-POU domain containing octamer binding antikoerper, non-POU domain containing, octamer-binding antikoerper, non-POU domain containing, octamer binding L homeolog antikoerper, non-POU-domain-containing, octamer binding protein antikoerper, NONO antikoerper, Nono antikoerper, nono.L antikoerper

Hintergrund: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

Molekulargewicht: 52 kDa

Gen-ID: 4841

NCBI Accession: NP_031389

UniProt: Q15233