NFYC Antikörper

Produktnummer ABIN2461844

Der Kaninchen Polyklonal Anti-NFYC-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, NFYC in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für NFYC Antikörper (ABIN2461844)

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFYC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-NFYC Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYC.

Anwendungsinformationen

Applikationshinweise: NFYC antibody can be used for detection of NFYC by ELISA at 1:62500. NFYC antibody can be used for detection of NFYC by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYC antibody at -20 °C.

Details zu NFYC

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Andere Bezeichnung: NFYC

Hintergrund: NFYC is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYC forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Molekulargewicht: 37 kDa

Gen-ID: 4802

NCBI Accession: NP_055038

UniProt: Q13952

Pathways: Regulation of Lipid Metabolism by PPARalpha