EGR4 Antikörper

Produktnummer ABIN2461643

Der Kaninchen Polyklonal Anti-EGR4-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, EGR4 in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für EGR4 Antikörper (ABIN2461643)

Target: EGR4 (Early Growth Response 4 (EGR4))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EGR4 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGR4.

Anwendungsinformationen

Applikationshinweise: EGR4 antibody can be used for detection of EGR4 by ELISA at 1:312500. EGR4 antibody can be used for detection of EGR4 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EGR4 antibody at -20 °C.

Details zu EGR4

Target: EGR4 (Early Growth Response 4 (EGR4))

Andere Bezeichnung: EGR4

Hintergrund: The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.

Molekulargewicht: 51 kDa

Gen-ID: 1961

NCBI Accession: NP_001956

UniProt: Q05215