MCM4 Antikörper

Produktnummer ABIN2461398

Dieses Anti-MCM4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MCM4 in WB und ELISA. Geeignet für Human.

Kurzübersicht für MCM4 Antikörper (ABIN2461398)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MCM4 Antikörper

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.

Anwendungsinformationen

Applikationshinweise: MCM4 antibody can be used for detection of MCM4 by ELISA at 1:62500. MCM4 antibody can be used for detection of MCM4 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MCM4 antibody at -20 °C.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Molekulargewicht: 95 kDa, 97 kDa

Gen-ID: 4173

NCBI Accession: NP_877423

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA