LEF1 Antikörper

Produktnummer ABIN2460458

Der Kaninchen Polyklonal Anti-LEF1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, LEF1 in Proben von Human, Maus, Ratte, Hund und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für LEF1 Antikörper (ABIN2460458)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reaktivität: Human, Maus, Ratte, Hund, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LEF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-LEF1 Antikörper

Aufreinigung: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.

Anwendungsinformationen

Applikationshinweise: LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store LEF1 antibody at -20 °C.

Details zu LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Andere Bezeichnung: LEF1

Hintergrund: Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1.

Molekulargewicht: 44 kDa

Gen-ID: 51176

NCBI Accession: NP_057353

UniProt: Q9UJU2

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding