INSIG1 Antikörper

Produktnummer ABIN2458475

Der Kaninchen Polyklonal Anti-INSIG1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, INSIG1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für INSIG1 Antikörper (ABIN2458475)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser INSIG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-INSIG1 Antikörper

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human INSIG1.

Anwendungsinformationen

Applikationshinweise: INSIG1 antibody can be used for detection of INSIG1 by ELISA at 1:1562500. INSIG1 antibody can be used for detection of INSIG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store INSIG1 antibody at -20 °C.

Details zu INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Andere Bezeichnung: INSIG1

Hintergrund: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molekulargewicht: 25 kDa

Gen-ID: 51141

NCBI Accession: NP_057217

UniProt: Q9Y5U4

Pathways: ER-Nucleus Signaling