SARS-CoV Spike Antikörper

Produktnummer ABIN2452119

Zitiert in 5+ Publikationen. Der Maus Monoklonal anti-SARS-CoV Spike Antikörper (Klon 3A2) (ABIN2452119) detektiert spezifisch SARS-CoV Spike in ELISA, WB, IF und IC. Dieser Antikörper reagiert spezifisch mit Proben aus SARS Coronavirus (SARS-CoV).

Kurzübersicht für SARS-CoV Spike Antikörper (ABIN2452119)

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Reaktivität: SARS Coronavirus (SARS-CoV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SARS-CoV Spike Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Klon: 3A2

Produktdetails anti-SARS-CoV Spike Antikörper

Produktmerkmale: Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids, which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.

Aufreinigung: Purified

Sterilität: Sterile filtered

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 0. 1~0. 3 g/mL
2) Immunofluoresece staining (IHC)
3) ELISA Isoform: IgG2b (kappa)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -80 C)

Referenzen für anti-SARS-CoV Spike Antikörper (ABIN2452119)

Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

Satija, Lal: "The molecular biology of SARS coronavirus." in: Annals of the New York Academy of Sciences, Vol. 1102, pp. 26-38, (2007) (PubMed).

Yamate, Yamashita, Goto, Tsuji, Li, Warachit, Yunoki, Ikuta: "Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus." in: Microbes and infection / Institut Pasteur, Vol. 7, Issue 15, pp. 1530-40, (2005) (PubMed).

Details zu SARS-CoV Spike

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Andere Bezeichnung: SARS-CoV Spike

Substanzklasse: Viral Protein

Hintergrund: A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor, ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by injecting mouse with SARS virus and as the clone which produces antibody that specifically reacts with the virus-infected cell, in the laboratory of Prof. K. Ikuta of Osaka University.