Influenza Nucleoprotein Antikörper (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2)

Produktnummer ABIN2452039

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in ELISA, WB, IF, IHC, IP und IC. Er zeigt eine Reaktivität gegenüber Influenza A Virus H2N2 und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Influenza Nucleoprotein Antikörper (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2) (ABIN2452039)

Target: Influenza Nucleoprotein (NP)

Reaktivität: Influenza A Virus H2N2

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunochromatography (IC)

Klon: C43

Produktdetails

Bindungsspezifität: H1N1, H2N2, H3N2, H5N1, H5N2

Spezifität: Reacts with NP of all influenza A viruses, including seasonal H2N2, H3N2, and avian H5N1, H5N2 and H1N1 (seasonal, pandemic and swine).

Kreuzreaktivität (Details): No cross reactivity with influenza B viruses.

Aufreinigung: Purified by proprietary chromatography procedure under mild conditions.

Reinheit: 90-95 % pure by SDS-PAGE

Sterilität: Sterile filtered

Immunogen: Human Influenza A Virus (H2N2) Okada strain

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 300~1,000 fold dilution
2) Immuno-precipitation (100 fold dilution
3) Immunofluorescent staining (200 fold dilution
4) Immunohistochemistry (200 fold dilution
4) ELISA (assay dependent)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Referenzen

Mizuike, Sasaki, Baba, Iwamoto, Shibai, Kosaka, Kubota-Koketsu, Yang, Du, Sakudo, Tsujikawa, Yunoki, Ikuta: "Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1." in: Clinical and vaccine immunology : CVI, Vol. 18, Issue 3, pp. 494-9, (2011) (PubMed).

Ueda, Yamate, Du, Daidoji, Okuno, Ikuta, Nakaya: "Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus." in: Virus research, Vol. 136, Issue 1-2, pp. 91-7, (2008) (PubMed).

Okuno, Isegawa, Sasao, Ueda: "A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains." in: Journal of virology, Vol. 67, Issue 5, pp. 2552-8, (1993) (PubMed).

Antigendetails

Target: Influenza Nucleoprotein (NP)

Andere Bezeichnung: Influenza A (Nucleoprotein)

Substanzklasse: Influenza Protein

Hintergrund: Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae. Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in the form of ribonucleoprotein complexes. Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA and is essential for RNA transcription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be essential for the viral lifecycle.