CBX3 Antikörper (C-Term)

Produktnummer ABIN2452035

Dieses Anti-CBX3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CBX3 in WB, IF und ChIP-seq. Geeignet für Human und Hamster. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für CBX3 Antikörper (C-Term) (ABIN2452035)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reaktivität: Human, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-CBX3 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität (Details): Expected to react with chicken, Xenopus, Drosophila, and zebra fish orthologs due to the sequence identity of the immunogen.

Produktmerkmale: The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ and purified by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.

Aufreinigung: Affinity purified

Sterilität: Sterile filtered

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1/2,000~1/10,000
2) Immunofluorescence staining
3) Chromatin immunoprecipitation (ChIP)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.75 mg/mL

Buffer: 0.12 M sodium phosphate buffer ( pH 7.4), 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -70 C)

Referenzen für anti-CBX3 Antikörper (ABIN2452035)

Lomberk, Wallrath, Urrutia: "The Heterochromatin Protein 1 family." in: Genome biology, Vol. 7, Issue 7, pp. 228, (2007) (PubMed).

Wang, Koyama, Nishida, Haraguchi, Reith, Tsukamoto: "The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4028-40, (2006) (PubMed).

Kametaka, Takagi, Hayakawa, Haraguchi, Hiraoka, Yoneda: "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1231-42, (2002) (PubMed).

Details zu CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Andere Bezeichnung: HP1gamma/CBX3

Hintergrund: Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene. HP1γ has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions.

UniProt: Q13185