HBEGF Antikörper (EGF Like Domain) (Biotin)

Produktnummer ABIN2451994

Dieser Maus Monoklonal Antikörper detektiert spezifisch HBEGF in WB, ELISA, IP, ICC und IF. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für HBEGF Antikörper (EGF Like Domain) (Biotin) (ABIN2451994)

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HBEGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 4G10

Produktdetails anti-HBEGF Antikörper (Biotin)

Bindungsspezifität: EGF Like Domain

Kreuzreaktivität (Details): Does not react with mouse.

Produktmerkmale: biotin/IgG = 7.5

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: Recombinant human HB-EGF ectodomain expressed in SF21 cell

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 0. 2~1 g/mL
2) Immunoprecipitation: 2 g/mL
3) Indirect immuno-fluorescence staining: 5~10 g/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -80 C)

Referenzen für anti-HBEGF Antikörper (Biotin) (ABIN2451994)

Miyamoto, Hirata, Yamazaki, Kageyama, Hasuwa, Mizushima, Tanaka, Yagi, Sonoda, Kai, Kanoh, Nakano, Mekada: "Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy." in: Cancer research, Vol. 64, Issue 16, pp. 5720-7, (2004) (PubMed).

Prenzel, Zwick, Daub, Leserer, Abraham, Wallasch, Ullrich: "EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF." in: Nature, Vol. 402, Issue 6764, pp. 884-8, (2000) (PubMed).

Iwamoto, Higashiyama, Mitamura, Taniguchi, Klagsbrun, Mekada et al.: "Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin ..." in: The EMBO journal, Vol. 13, Issue 10, pp. 2322-30, (1994) (PubMed).

Higashiyama, Abraham, Miller, Fiddes, Klagsbrun: "A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF." in: Science (New York, N.Y.), Vol. 251, Issue 4996, pp. 936-9, (1991) (PubMed).

Details zu HBEGF

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Andere Bezeichnung: HB-EGF

Hintergrund: Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a membrane-anchored precursor that is proteolytically cleaved to release the soluble mature growth factor, HB-EGF. The former functions as juxtacrine and the latter as paracrine growth factor. Soluble HB-EGF shows several forms in Western blotting with apparent molecular weights 19~27 kDa due to heterogeneous O-glycosylation and N-terminal truncation. HB-EGF activates EGFR and ErbB4 and promotes the development in many tissues. In human ProHB-EGF is the cellular receptor for diphtheria toxin. Non-toxic mutant of diphtheria toxin, CRM197, inhibits HB-EGF function. As HB-EGF level is elevated in most ovarian cancer, CRM197 is being tested as an anticancer drug. The hybridoma clone 4G10 was established and characterized in the laboratory of Prof. E. Mekada of Osaka University, who is a leading scientist in this field.

UniProt: Q99075

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung