GAPDH Antikörper

Produktnummer ABIN2443912

Dieser Maus Monoklonal Antikörper detektiert spezifisch GAPDH in WB, ELISA, IHC und DB. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Huhn, Kaninchen, Hamster, Saccharomyces cerevisiae, E. coli und Sf9 insect cell line.

Kurzübersicht für GAPDH Antikörper (ABIN2443912)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Huhn, Kaninchen, Hamster, Saccharomyces cerevisiae, E. coli, Sf9 insect cell line

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Klon: GA1R

Produktdetails anti-GAPDH Antikörper

Verwendungszweck: GAPDH Mouse Monoclonal Antibody (GA1R)

Spezifität: The antibody recognizes native and denatured forms of GAPDH.

Produktmerkmale: Specific detection of E coli BL21

Aufreinigung: Protein G purified

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-2,000, IHC 1:500-2000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund:

Glyceraldehyde 3-phosphate dehydrogenase

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a 37KDa glycolytic enzyme constitutively and stably expressed in almost all tissues. This enzyme catalyzes the sixth step of glycolysis by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Proteins such as GAPDH involved in maintenance of basic cellular function are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

UniProt: P04406