beta Actin Antikörper

Produktnummer ABIN2443850

Der Maus Monoklonal anti-beta Actin Antikörper wird verwendet zum Nachweis von beta Actin in Proben von Human, Maus, Ratte, Huhn und Kaninchen. Er wurde validiert für WB, ELISA, IHC und DB.

Kurzübersicht für beta Actin Antikörper (ABIN2443850)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Huhn, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Klon: BA3R

Produktdetails anti-beta Actin Antikörper

Verwendungszweck: Beta-Actin Mouse Monoclonal Antibody (BA3R)

Spezifität: The antibody recognizes native and denatured forms of β-Actin (~42 kDa)

Aufreinigung: Protein G purified

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IHC 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Haltbarkeit: 24 months

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: Beta-Actin

Hintergrund: β-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The β-actin gene is often stably and constitutively expressed at high levels in most tissues and cells. Proteins such as β-actin, involved in maintenance of basic cellular function, are often referred to as housekeeping proteins. Housekeeping proteins are frequently used as loading controls for western blots and protein normalization.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction