TUBG1 Antikörper (C-Term)

Produktnummer ABIN238522

Produktdetails anti-TUBG1 Antikörper

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Bindungsspezifität: AA 434-449, C-Term

Reaktivität: Human, Ratte, Maus, Huhn, Pflanzen, Protozoa, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.

Produktmerkmale: Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

Aufreinigung: Protein-A Affinity Chromatography

Immunogen: C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ

Klon: TU-30

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry (Purified Antibody: 1-2 μg/mL, Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly inmethanol for 10 min at -20C or in acetone for 10 min at -20C. Incubation: 45 min in roomtemperature, Positive control: P-19 mouse embryonal carcinoma cell line 3T3 mousefibroblasts, Application note: The antibody TU-30 stains only fixed cells). Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Andere Bezeichnung: TUBG1 / Tubulin gamma 1 (TUBG1 Produkte)

Hintergrund: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

Gen-ID: 7283

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase