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TUBG1 Antikörper (C-Term)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TUBG1 in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Huhn, Pflanzen, Protozoa und Schwein.
Produktnummer ABIN238522

Kurzübersicht für TUBG1 Antikörper (C-Term) (ABIN238522)

Target

Alle TUBG1 Antikörper anzeigen
TUBG1 (Tubulin, gamma 1 (TUBG1))

Reaktivität

  • 57
  • 30
  • 26
  • 6
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Ratte, Maus, Huhn, Pflanzen, Protozoa, Schwein

Wirt

  • 43
  • 17
Maus

Klonalität

  • 40
  • 20
Monoklonal

Konjugat

  • 40
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser TUBG1 Antikörper ist unkonjugiert

Applikation

  • 42
  • 18
  • 12
  • 11
  • 10
  • 8
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Klon

TU-30
  • Bindungsspezifität

    • 8
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 434-449, C-Term

    Spezifität

    The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.

    Produktmerkmale

    Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1

    Aufreinigung

    Protein-A Affinity Chromatography

    Immunogen

    C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ

    Isotyp

    IgG1
  • Applikationshinweise

    Immunocytochemistry (Purified Antibody: 1-2 μg/mL, Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly inmethanol for 10 min at -20C or in acetone for 10 min at -20C. Incubation: 45 min in roomtemperature, Positive control: P-19 mouse embryonal carcinoma cell line 3T3 mousefibroblasts, Application note: The antibody TU-30 stains only fixed cells). Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
    Shelf life: one year from despatch.

    Haltbarkeit

    12 months
  • Target

    TUBG1 (Tubulin, gamma 1 (TUBG1))

    Andere Bezeichnung

    TUBG1 / Tubulin gamma 1

    Hintergrund

    The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

    Gen-ID

    7283

    UniProt

    P23258

    Pathways

    Microtubule Dynamics, M Phase
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