TUBB Antikörper

Produktnummer ABIN238518

Dieses Anti-TUBB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TUBB in WB und IF. Geeignet für Schwein. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für TUBB Antikörper (ABIN238518)

Target: TUBB (Tubulin, beta (TUBB))

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: TU-12

Produktdetails anti-TUBB Antikörper

Spezifität: The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.

Produktmerkmale: Synonyms: Tubulin beta chain, Tubulin beta-5 chain

Aufreinigung: Precipitation Methods.

Immunogen: Porcine brain microtubule protein MTP-1.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Western Blotting (Reducing conditions): Recommended dilution: 2 μg/mL, 60 min in room temperaturePositive Control: Porcine brain lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-TUBB Antikörper (ABIN238518)

Blume, Yemets, Sheremet, Nyporko, Sulimenko, Sulimenko, Dráber: "Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells." in: BMC plant biology, Vol. 10, pp. 29, (2010) (PubMed).

Details zu TUBB

Target: TUBB (Tubulin, beta (TUBB))

Andere Bezeichnung: TUBB / TUBB5

Hintergrund: The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.Synonyms: Tubulin beta chain, Tubulin beta-5 chain

Gen-ID: 203068

UniProt: P07437

Pathways: Microtubule Dynamics, M Phase