Clusterin Antikörper

Produktnummer ABIN238421

Der Maus Monoklonal anti-Clusterin Antikörper wird verwendet zum Nachweis von Clusterin in Proben von Human. Er wurde validiert für WB, IF, IHC (p) und EIA.

Kurzübersicht für Clusterin Antikörper (ABIN238421)

Target: Clusterin (CLU)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: Hs-3

Produktdetails anti-Clusterin Antikörper

Spezifität: Recognizes Apolipoprotein J (Apo J).

Kreuzreaktivität (Details): Species reactivity (tested):Human. Negative in Bovine, Canine (Dog) and Feline (Cat).

Produktmerkmale: Synonyms: Clusterin, APOJ, CLI, KUB1, AAG4, NA1/NA2, SP-40, 40, Complement-associated proteinSP-40, Complement cytolysis inhibitor, Testosterone-repressed prostate message 2,Ku70-binding protein 1, Aging-associated gene 4 protein

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% acetic acid and subsequently used forimmunization.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1/ μg/mLPositive Material: seminal plasmaImmunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Positive Tissue: Sertoli cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4 with 15 mM Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Apolipoprotein J (Apo J)

Hintergrund: Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gen-ID: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling