PSMA Antikörper (AA 44-750)

Produktnummer ABIN238408

Produktdetails anti-PSMA Antikörper

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Bindungsspezifität: AA 44-750

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PSMA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Spezifität: The antibody recognizes extracellular domain of Glutamate Carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen,FOLH, NAALAD1, PSM, GCP2, NAALAdase, Glutamate carboxypeptidase II, Membraneglutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I,Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamatecarboxypeptidase

Aufreinigung: Protein A Chromatography

Immunogen: Amino acids 44-750 of Human PSMA/GCPII

Klon: GCP-05

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunoprecipitation. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-PSMA Antikörper (ABIN238408)

Sácha, Zámecník, Barinka, Hlouchová, Vícha, Mlcochová, Hilgert, Eckschlager, Konvalinka: "Expression of glutamate carboxypeptidase II in human brain." in: Neuroscience, Vol. 144, Issue 4, pp. 1361-72, (2007) (PubMed).

Barinka, Mlcochová, Sácha, Hilgert, Majer, Slusher, Horejsí, Konvalinka: "Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding." in: European journal of biochemistry / FEBS, Vol. 271, Issue 13, pp. 2782-90, (2004) (PubMed).

Barinka, Sácha, Sklenár, Man, Bezouska, Slusher, Konvalinka: "Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 6, pp. 1627-35, (2004) (PubMed).

Details zu PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Andere Bezeichnung: PSMA / FOLH1 (FOLH1 Produkte)

Synonyme: FGCP antikoerper, FOLH antikoerper, GCP2 antikoerper, GCPII antikoerper, NAALAD1 antikoerper, NAALAdase antikoerper, PSM antikoerper, PSMA antikoerper, mGCP antikoerper, mopsm antikoerper, Naalad antikoerper, putative N-acetylated-alpha-linked acidic dipeptidase antikoerper, folate hydrolase 1B antikoerper, folate hydrolase 1 antikoerper, folate hydrolase (prostate-specific membrane antigen) 1 antikoerper, LOC451185 antikoerper, FOLH1B antikoerper, FOLH1 antikoerper, Folh1 antikoerper

Hintergrund: Glutamate Carboxypeptidase II (GCPII), also known as N-acetyl-a-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer's disease, Huntington's disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-g-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM') than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.Synonyms: FOLH, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD1, NAALAdase, PSM, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase

Gen-ID: 2346

UniProt: Q04609