alpha Tubulin Antikörper (Biotin)

Produktnummer ABIN2380025

Der Kaninchen Polyklonal Anti-alpha Tubulin-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, alpha Tubulin in Proben von Human zu detektieren.

Kurzübersicht für alpha Tubulin Antikörper (Biotin) (ABIN2380025)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Tubulin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-alpha Tubulin Antikörper (Biotin)

Kreuzreaktivität: Rind (Kuh), Huhn, Ziege, Human, Maus, Ratte (Rattus), Schaf

Kreuzreaktivität (Details): Calculated cross reactivity: Bo Ch Gt Hu Mo Rt Sh

Produktmerkmale: Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)

Aufreinigung: Purified by immunoaffinity chromatography.

Immunogen: Synthetic peptide corresponding to aa 425-451 at the C-Terminal of human alpha tubulin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 100ul sterile dH2O.

Buffer: Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: Tubulin, alpha

Hintergrund: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated a A tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.

Pathways: Microtubule Dynamics