Collagen Type V Antikörper

Produktnummer ABIN237044

Der Kaninchen Polyklonal anti-Collagen Type V Antikörper wird verwendet zum Nachweis von Collagen Type V in Proben von Human. Er wurde validiert für WB, ELISA und IHC (f).

Kurzübersicht für Collagen Type V Antikörper (ABIN237044)

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen Type V Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Collagen Type V Antikörper

Spezifität: Reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

Produktmerkmale: Rabbit anti Collagen Type V, Rabbit antibody to Human Collagen Type V

Aufreinigung: Immuno Affinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen: Collagen Type V from human and bovine placenta

Anwendungsinformationen

Applikationshinweise: Have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve and for non-denaturing immunoblot for highly sensitive qualitative analysis. Was assayed by non-denaturing immunoblot and found to be reactive against Collagen V at a dilution of 1:5.000 to 1:10.000. This product was also assayed against 1.0 µg of Collagen V in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG (H&L) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:8.000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt, store at 2-8 °C as an undiluted liquid. For long term storage, mix with an equal volume of glycerol, aliquot and store at -28 °C.

Haltbarkeit: 12 months

Details zu Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Andere Bezeichnung: Collagen Type V