ODF2 Antikörper (pSer796)

Produktnummer ABIN233845

Hersteller Produkt- Nr.: 600-401-a47

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ODF2 in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human, Schimpanse und Makake.

Kurzübersicht für ODF2 Antikörper (pSer796) (ABIN233845)

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Reaktivität: Human, Schimpanse, Makake

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ODF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-ODF2 Antikörper

Bindungsspezifität: pSer796

Hersteller Produkt- Nr.: 600-401-a47

Hersteller: Rockland

Verwendungszweck: Cenexin-1 phospho S796 Antibody

Kreuzreaktivität (Details): This antibody is specific for human Cenexin-1 protein phosphorylated at S796.

Produktmerkmale: Synonyms: rabbit anti-Cenexin-1 pS796 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2, Cenexin1 variant 1 antibody, KKT 4 antibody, ODF2, Cenexin 1, Cenexin-1, Outer dense fiber protein 2

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Andere Bezeichnung: ODF2

Hintergrund: Background: Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation.  Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene, however, not all isoforms and variants have been fully described.

Gen-ID: 4957

NCBI Accession: NP_702915

UniProt: Q5BJF6

Pathways: M Phase