CENPA Antikörper (AA 4-17)
Kurzübersicht für CENPA Antikörper (AA 4-17) (ABIN232755)
Target
Alle CENPA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 4-17
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Spezifität
- Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
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Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Rabbit (93%) Nectri (86%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Rabbit (93%), Nectri (86%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
Isotyp
- IgG
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Applikationshinweise
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Approved: WB
Usage: Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 μg control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 μg/mL anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 5 mg/mL BSA, 0.05 % sodium azide, 30 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CENPA (Centromere Protein A (CENPA))
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Andere Bezeichnung
- CENPA / CENP-A
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Hintergrund
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Name/Gene ID: CENPA
Synonyms: CENPA, Centromere autoantigen A, Centromere-specific histone, CenH3, Centromere protein A, Centromere protein A, 17 kDa, CENP-A, Centromere protein A (17kD) -
Gen-ID
- 1058
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UniProt
- P49450
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Pathways
- Chromatin Binding, Maintenance of Protein Location
Target
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