GNAS Antikörper (AA 1028-1037)

Produktnummer ABIN228262

Produktdetails anti-GNAS Antikörper

Target: GNAS (GNAS Complex Locus (GNAS))

Bindungsspezifität: AA 1028-1037

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein, Pferd, Affe, Hamster, Fledermaus, Zebrafisch (Danio rerio), Huhn, Drosophila melanogaster, Insekten, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Spezifität: Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to the C-terminal region of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:100), IP, WB (1:500 - 1:1000)

Usage: Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20 μg of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2 μL immunoprecipitates Gs alpha(45kD) from 500 μg of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100 % ice-cold acetone.Optimal dilutions to be determined by researcher.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Andere Bezeichnung: GNAS (GNAS Produkte)

Synonyme: AHO antikoerper, C20orf45 antikoerper, GNAS1 antikoerper, GPSA antikoerper, GSA antikoerper, GSP antikoerper, NESP antikoerper, PHP1A antikoerper, PHP1B antikoerper, PHP1C antikoerper, POH antikoerper, GNAS antikoerper, PORGSA1 antikoerper, G-alpha-S antikoerper, 5530400H20Rik antikoerper, A930027G11Rik antikoerper, C130027O20Rik antikoerper, Galphas antikoerper, Gnas1 antikoerper, Gnasxl antikoerper, Gsa antikoerper, Nesp antikoerper, Nespl antikoerper, Oed-Sml antikoerper, Oedsml antikoerper, P1 antikoerper, P2 antikoerper, P3 antikoerper, G-alpha-s antikoerper, gnas-a antikoerper, Gnpas antikoerper, Nesp55 antikoerper, gnal antikoerper, Gnas antikoerper, GNAS complex locus antikoerper, guanine nucleotide-binding protein G(s) subunit alpha antikoerper, GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus antikoerper, GNAS complex locus S homeolog antikoerper, neuroendocrine secretory protein 55-like antikoerper, GNAS antikoerper, LOC694289 antikoerper, LOC469986 antikoerper, Gnas antikoerper, gnas.S antikoerper, gnas antikoerper, LOC100346323 antikoerper, LOC100442753 antikoerper, LOC100732436 antikoerper

Hintergrund: Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI

Gen-ID: 2778

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis