GNAS Antikörper (AA 1028-1037)

Produktnummer ABIN228262

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GNAS in WB, ICC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Rind (Kuh), Schwein, Pferd, Affe, Fledermaus, Zebrafisch (Danio rerio), Hamster, Huhn, Drosophila melanogaster, Insekten und Xenopus laevis.

Kurzübersicht für GNAS Antikörper (AA 1028-1037) (ABIN228262)

Target: GNAS (GNAS Complex Locus (GNAS))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Schwein, Pferd, Affe, Fledermaus, Zebrafisch (Danio rerio), Hamster, Huhn, Drosophila melanogaster, Insekten, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GNAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-GNAS Antikörper

Bindungsspezifität: AA 1028-1037

Spezifität: Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to the C-terminal region of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:100), IP, WB (1:500 - 1:1000)

Usage: Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20 μg of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2 μL immunoprecipitates Gs alpha(45kD) from 500 μg of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100 % ice-cold acetone.Optimal dilutions to be determined by researcher.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Andere Bezeichnung: GNAS

Hintergrund: Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI

Gen-ID: 2778

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis