Choline Acetyltransferase Antikörper

Produktnummer ABIN224453

Der Maus Monoklonal anti-Choline Acetyltransferase Antikörper wird verwendet zum Nachweis von Choline Acetyltransferase in Proben von Human, Ratte und Schwein. Er wurde validiert für WB, IHC, ELISA und IHC (pfa).

Kurzübersicht für Choline Acetyltransferase Antikörper (ABIN224453)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Human, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Klon: 1-B3-9B3

Produktdetails anti-Choline Acetyltransferase Antikörper

Spezifität: Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.

Aufreinigung: Purified

Immunogen: Highly purified ChAT obtained from porcine brain

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Pfa (10 - 20 μg/mL), WB

Usage: Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4 % paraformaldehyde/10 % formaldehyde for 1 week at 4 °C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10 %, 20 % and 30 %). Prepare 50um sections and store in a cryoprotective solution at -15 °C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.

Kommentare:

Target Species of Antibody: Pig

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: CHAT

Hintergrund: Name/Gene ID: CHAT

Synonyms: CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A

Gen-ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development