Vimentin Antikörper (FITC)

Produktnummer ABIN222825

Der FITC-konjugierte Maus Monoklonal anti-Vimentin Antikörper (ABIN222825) detektiert spezifisch Vimentin in WB, IHC, ELISA, IF, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Rind (Kuh), Affe, Huhn und Hund.

Kurzübersicht für Vimentin Antikörper (FITC) (ABIN222825)

Target: Vimentin (VIM)

Reaktivität: Human, Rind (Kuh), Affe, Huhn, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Vimentin Antikörper (FITC)

Spezifität: Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57000 intermediate filament protein (vimentin) of mesenchymal cells. Reactivities on Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Species cross-reactivity: Human, monkey, bovine, canine, chicken, amphibia.

Aufreinigung: Protein A purified

Immunogen: Vimentin (purified from bovine lens).

Type of Immunogen: Purified protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Western Blot, ELISA, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT, extended with paraffin. Protease pretreatment is required prior to antibody application. Positive control: Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as a liquid.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose