Serotonin Receptor 5A Antikörper (AA 17-34)

Produktnummer ABIN222307

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Serotonin Receptor 5A in WB, IF und IHC. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Serotonin Receptor 5A Antikörper (AA 17-34) (ABIN222307)

Target: Serotonin Receptor 5A (HTR5A)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Serotonin Receptor 5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-Serotonin Receptor 5A Antikörper

Bindungsspezifität: AA 17-34

Spezifität: Recognizes Serotonin Receptor 5A. Species cross-reactivity: Mouse and rat.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide sequence corresponding to aa17-34 of the rat 5-HT5A receptor.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC (1:100 - 1:300), WB (1:100 - 1:250)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS/0.03 % Triton X-100 - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and hippocampus. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation: 4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4, 500 mL over approximately 20 minutes. Post Fixation -1.5 hour at 4? C. in 4 % paraformaldehyde in 0.1 M phosphate buffer,  pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3 %) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Absorption control: Synthetic rat 5HT 5A receptor. Incubation on Tissue: 16-24 hours at 4?C.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as a liquid.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Andere Bezeichnung: HTR5A / 5-HT5A Receptor

Hintergrund: Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A

Gen-ID: 3361

UniProt: P47898

Pathways: JAK-STAT Signalweg