Prohibitin Antikörper

Produktnummer ABIN221967

Dieses Anti-Prohibitin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Prohibitin in WB, IHC, IF, IHC (p) und IP. Geeignet für Human, Maus, Ratte, Schwein und Huhn.

Kurzübersicht für Prohibitin Antikörper (ABIN221967)

Target: Prohibitin (PHB)

Reaktivität: Human, Maus, Ratte, Schwein, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prohibitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-Prohibitin Antikörper

Spezifität: Recognizes rat Prohibitin at 30kD. Species cross-reactivity: Human, porcine, mouse and chicken.

Aufreinigung: Protein G purified

Immunogen: Purified recombinant rat prohibitin protein. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate., (Protein G). Native and denatured. Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: MAD109, or PC12 cells, heart.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Prohibitin

Target: Prohibitin (PHB)

Andere Bezeichnung: PHB / Prohibitin

Hintergrund: Name/Gene ID: PHB

Synonyms: PHB, PHB1, Prohibitin

Gen-ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling