Leptin Receptor Antikörper (Soluble)

Produktnummer ABIN2192046

Dieses Anti-Leptin Receptor-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Leptin Receptor in WB, IHC (p), IHC (fro) und IA. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Leptin Receptor Antikörper (Soluble) (ABIN2192046)

Target: Leptin Receptor (LEPR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Leptin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Klon: 2F1

Produktdetails anti-Leptin Receptor Antikörper

Bindungsspezifität: Soluble

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For Western blot, immuno assays and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Leptin Receptor Antikörper (ABIN2192046)

van Dielen, van t Veer, Buurman, Greve: "Leptin and soluble leptin receptor levels in obese and weight-losing individuals." in: The Journal of clinical endocrinology and metabolism, Vol. 87, Issue 4, pp. 1708-16, (2002) (PubMed).

Details zu Leptin Receptor

Target: Leptin Receptor (LEPR)

Hintergrund: The monoclonal antibody 2F1 reacts with human soluble leptin receptor (sLR) in plasma. Leptin is a cytokine that is primarily expressed by adipose tissue. Leptin controls food intake by its interaction with the leptin receptor in the brain. Leptin action is mediated and controlled by the leptin receptor, a class I type cytokine receptor. sLR is generated by proteolytic cleavage of membrane-anchored receptors. This indicates that the leptin receptor might have other functions besides transduction. Binding of leptin with sLR increases the bioavailability of leptin in plasma, but also decreases the binding of leptin to membrane bound leptin receptors. For example, when comparing obese and lean individuals, plasma levels of sLR are significantly decreased whereas leptin levels are significantly increased. The monoclonal antibody 2F1 specifically reacts with sLR with a molecular mass of 180kD. The 2F1 antibody can be used to measure in both free sLR and sLR bound to leptin in plasma.

Pathways: JAK-STAT Signalweg, AMPK Signaling, Feeding Behaviour