MASP1 Antikörper

Produktnummer ABIN2191968

Dieses Anti-MASP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MASP1 in WB, IP und IA. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für MASP1 Antikörper (ABIN2191968)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MASP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)

Klon: 1E2

Produktdetails anti-MASP1 Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. The antibody does not work in Western blotting of reduced samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-MASP1 Antikörper (ABIN2191968)

Hajela, Kojima, Ambrus, Wong, Moffatt, Ferluga, Hajela, Gál, Sim: "The biological functions of MBL-associated serine proteases (MASPs)." in: Immunobiology, Vol. 205, Issue 4-5, pp. 467-75, (2002) (PubMed).

Endo, Takahashi, Kuraya, Matsushita, Stover, Schwaeble, Fujita: "Functional characterization of human mannose-binding lectin-associated serine protease (MASP)-1/3 and MASP-2 promoters, and comparison with the C1s promoter." in: International immunology, Vol. 14, Issue 10, pp. 1193-201, (2002) (PubMed).

Kuraya, Matsushita, Endo, Thiel, Fujita: "Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G." in: International immunology, Vol. 15, Issue 1, pp. 109-17, (2002) (PubMed).

Matsushita, Kuraya, Hamasaki, Tsujimura, Shiraki, Fujita: "Activation of the lectin complement pathway by H-ficolin (Hakata antigen)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3502-6, (2002) (PubMed).

Matsushita, Endo, Hamasaki, Fujita: "Activation of the lectin complement pathway by ficolins." in: International immunopharmacology, Vol. 1, Issue 3, pp. 359-63, (2001) (PubMed).

Details zu MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Andere Bezeichnung: Masp-1/3

Hintergrund: Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component C3. In the lectin pathway, mannose binding-lectin (MBL)-associated serine proteases (MASP) form complexes with polymeric lectin molecules which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of microorganisms, MASP are converted to their active forms and initiate complement activation. Three types of human MASP have been reported. MASP- 1, MASP-2 and MASP-3. MASP-1 appears to cleave the second complement component C2, but not C4. The proteolytic activities of MASP-1 are inhibited by C1-inhibitor. Furthermore MASP-1 has a reactivity profile very similar to that of thrombin. MASP-1 is able to catalyse the formation of cross-linked fibrin. Participation of MASP-1 in cross- linked fibrin clot formation causes release of a chemotactic factor representing a biologically significant activity of MASP-1. The alternative-splicing product from MASP-1 gene is called MASP-3. MASP-1 is associated with smaller MBL oligomers whereas MASP-3 is found on larger oligomers. The substrate of MASP-3 is unknown. The antibody recognizes the heavy chain common to both MASP-1 and MASP-3.

Pathways: Komplementsystem