Polymyxin B Antikörper

Produktnummer ABIN2191915

Produktdetails anti-Polymyxin B Antikörper

Target: Polymyxin B

Reaktivität: Chemical

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Polymyxin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Endotoxin-Niveau: Low endotoxin level

Klon: 45

Anwendungsinformationen

Applikationshinweise: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Polymyxin B Antikörper (ABIN2191915)

Appelmelk, Su, Verweij-van Vught, Thijs, MacLaren: "Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research." in: Analytical biochemistry, Vol. 207, Issue 2, pp. 311-6, (1993) (PubMed).

Details zu Polymyxin B

Target: Polymyxin B

Abstract: Polymyxin B Produkte

Substanzklasse: Chemical

Hintergrund: The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.