Interferon gamma Antikörper

Produktnummer ABIN2191865

Produktdetails anti-Interferon gamma Antikörper

Target: Interferon gamma (IFNG)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Interferon gamma Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Klon: F12

Anwendungsinformationen

Applikationshinweise: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23- 901-530).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the vial by injection of 1 mLdistilled or de-ionized water (Caution: vial is under vacuum).

Buffer: Lyophilized product in PBS, containing 300 μg antibody.

Handhabung: Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.

Lagerung: 4 °C

Informationen zur Lagerung: Lyophilized product should be stored at 4 °C Store stock solution in aliquots at -20 °C Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Interferon gamma Antikörper (ABIN2191865)

Billiau: "Gamma-interferon: the match that lights the fire?" in: Immunology today, Vol. 9, Issue 2, pp. 37-40, (1989) (PubMed).

Jacob, van der Meide, McDevitt: "In vivo treatment of (NZB X NZW)F1 lupus-like nephritis with monoclonal antibody to gamma interferon." in: The Journal of experimental medicine, Vol. 166, Issue 3, pp. 798-803, (1987) (PubMed).

Van der Meide, Dubbeld, Schellekens: "Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA." in: Journal of immunological methods, Vol. 79, Issue 2, pp. 293-305, (1985) (PubMed).

Armstrong: "Semi-micro, dye-binding assay for rabbit interferon." in: Applied microbiology, Vol. 21, Issue 4, pp. 723-5, (1971) (PubMed).

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: gamma Interferon (IFNG Produkte)

Hintergrund: Monoclonal antibody binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. The antibody does not react with rodent interferons. Possible applications include the purification of human gamma Interferon and in vitro neutralisation. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie