ErbB2/Her2 Antikörper (Cytoplasmic Domain)
Kurzübersicht für ErbB2/Her2 Antikörper (Cytoplasmic Domain) (ABIN219136)
Target
Alle ErbB2/Her2 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Bindungsspezifität
- Cytoplasmic Domain
-
Spezifität
- Recognizes human c-erbB-2 (HER-2, neu). Species cross-reactivity: Mouse and rat.
-
Aufreinigung
- Protein G purified
-
Immunogen
- Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein. MW of Antigen: 185kD. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane.
-
Isotyp
- IgG1
-
-
-
-
Applikationshinweise
-
Approved: IHC (2 - 4 μg/mL), IP, WB (1 - 2 μg/mL)
Usage: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 μg/mg protein lysate. Western Blot: 1-2 μg/mL for 2hr at RT. Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma. -
Kommentare
-
Target Species of Antibody: Human
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- Lot specific
-
Buffer
- PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
-
Andere Bezeichnung
- ERBB2 / HER2
-
Hintergrund
-
Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 -
Gen-ID
- 2064
-
UniProt
- P04626
-
Pathways
- RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development
Target
-