IL-4 Antikörper (Biotin)

Produktnummer ABIN218108

Der Ratte Monoklonal Anti-IL-4-Antikörper wurde für WB, ELISA, FACS und IF validiert. Er ist geeignet, IL-4 in Proben von Human zu detektieren.

Kurzübersicht für IL-4 Antikörper (Biotin) (ABIN218108)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-4 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-IL-4 Antikörper (Biotin)

Spezifität: Recognizes human Interleukin-4 (IL-4). Has been reported to cross-react with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.

Aufreinigung: Purified

Immunogen: CHO-expressed recombinant human IL-4.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IF, WB

Usage: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/. Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-4 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL4

Hintergrund: Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

Gen-ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation