Nanog Antikörper (AA 101-200)

Produktnummer ABIN2177828

Der Kaninchen Polyklonal Anti-Nanog-Antikörper wurde für WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, Nanog in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Nanog Antikörper (AA 101-200) (ABIN2177828)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Nanog Antikörper

Bindungsspezifität: AA 101-200

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Nanog

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Nanog Antikörper (ABIN2177828)

Nasr, Farghaly, Elsaba, El-Mokhtar, Radwan, Elsabahy, Abdelkareem, Fakhry, Mousa: "Resistance of primary breast cancer cells with enhanced pluripotency and stem cell activity to sex hormonal stimulation and suppression." in: The international journal of biochemistry & cell biology, Vol. 105, pp. 84-93, (2019) (PubMed).

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: Nanog

Hintergrund:

Synonyms: ENK, ecat4, 2410002E02Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Gen-ID: 71950

UniProt: Q80Z64

Pathways: Stem Cell Maintenance