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GPR84 Antikörper (AA 21-120)

Name: GPR84 Antikörper (AA 21-120)
SKU: ABIN2173922
Price: 357.7 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-GPR84-Antikörper wurde für WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, GPR84 in Proben von Human, Maus und Kaninchen zu detektieren.

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 Antikörper (AA 21-120))

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 Antikörper (AA 21-120))

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (GPR84 Antikörper (AA 21-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (GPR84 Antikörper (AA 21-120))

IHC (p)

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (GPR84 Antikörper (AA 21-120))

FACS

5 Abbildungen

Produktnummer ABIN2173922

Kurzübersicht für GPR84 Antikörper (AA 21-120) (ABIN2173922)

Target

Alle GPR84 Antikörper anzeigen

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reaktivität

Human, Maus, Kaninchen

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser GPR84 Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Bindungsspezifität
16

7

5

3

3

2

2

1

1

1

1

1

1

1

1

1
AA 21-120

Kreuzreaktivität

Human, Maus, Kaninchen

Homologie

Cow,Pig

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

Isotyp

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 Reaktivität: Human ELISA, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 166-180), (Extracellular) antibody
GPR84 Reaktivität: Human WB, FACS, IHC, LCI, IF Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 Reaktivität: Human IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 Reaktivität: Human, Pferd, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 Reaktivität: Human, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 Reaktivität: Human ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 Reaktivität: Maus WB, ELISA, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 265-293) antibody
GPR84 Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB36053 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reaktivität: Human WB, ELISA Wirt: Maus Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Andere Bezeichnung

GPCR EX33

Hintergrund

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

Gen-ID

53831

UniProt

Q9NQS5