beta Actin Antikörper (N-Term)

Produktnummer ABIN215582

Der Kaninchen-Polyklonal Anti-beta Actin-Antikörper wurde für WB, IF, ICC und IP validiert. Er ist geeignet zum Nachweis von beta Actin in Proben aus Human, Maus, Ratte und Affe. }

Kurzübersicht für beta Actin Antikörper (N-Term) (ABIN215582)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes human beta Actin. Species cross-reactivity: mouse, rat, monkey. Expected to cross-react with a wide range of species due to sequence homology.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IF (1:50), IP, WB (1:500 - 1:2500)

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control. Immunofluorescence: 1:50. Positive control: HeLa whole cell lysate and 3T3 cells (mouse).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: ACTB / Beta Actin

Hintergrund: Name/Gene ID: ACTB

Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1

Gen-ID: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction