HIF3A Antikörper (AA 581-592)

Produktnummer ABIN214599

Produktdetails anti-HIF3A Antikörper

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Bindungsspezifität: AA 581-592

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIF3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Hif3 a (Hypoxia Inducible Factor).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from mouse HIF3A.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:14000 - 1:80000), IF, IHC, IHC-P (5 μg/mL), WB (1:100 - 1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.***Does not work to detect human protein in Western blot.***

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Andere Bezeichnung: HIF3A / HIF3-Alpha (HIF3A Produkte)

Hintergrund: Name/Gene ID: HIF3A

Synonyms: HIF3A, BHLHe17, HIF-3A, IPAS, HIF3-alpha-1, Inhibitory PAS domain protein, PASD7, HIF-3-alpha, HIF3-alpha, Member of PAS protein 7, MOP7

Gen-ID: 64344

UniProt: Q9Y2N7

Pathways: Warburg Effekt