SGK1 Antikörper (AA 419-431)

Produktnummer ABIN214577

Der Kaninchen Polyklonal Anti-SGK1-Antikörper wurde für WB, ELISA, IHC und IHC (p) validiert. Er ist geeignet, SGK1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für SGK1 Antikörper (AA 419-431) (ABIN214577)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SGK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SGK1 Antikörper

Bindungsspezifität: AA 419-431

Marke: IHC-plus™

Spezifität: Amino acids 419-431 of human SGK -1.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human SGK1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:4000 - 1:16000), IHC, IHC-P (10 - 20 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10-20 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Andere Bezeichnung: SGK1 / SGK

Hintergrund: Name/Gene ID: SGK1
Subfamily: SGK
Family: Protein Kinase

Synonyms: SGK1, Hsgk, Hsgk1, SGK, Sgk1 variant i3

Gen-ID: 6446

UniProt: O00141

Pathways: MAPK Signalweg, Notch Signalweg, Steroid Hormone Mediated Signaling Pathway