CD47 Antikörper (PE)

Produktnummer ABIN2144904

Dieses Anti-CD47-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD47 in FACS. Geeignet für Human.

Kurzübersicht für CD47 Antikörper (PE) (ABIN2144904)

Target: CD47

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: 4ACC2C6D4

Produktdetails anti-CD47 Antikörper (PE)

Produktmerkmale: 4ACC2C6D4 reacts with CD47 ( gp42 ), a 45-55 kDa molecule, expressed on broad tissue and cells including hemopoietic cells , epithelial, endothelial cells and other tissue cells. CD47 antigen function on adhesion molecule and thrombospondin receptor.This clone is cross reactive with non-human primate.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47