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CD45RA Antikörper (FITC)

This anti-CD45RA antibody (ABIN2144603) is a Mouse Monoclonal antibody detecting CD45RA in FACS, IF, IHC (p), WB. Suitable for Human.
Produktnummer ABIN2144603

Quick Overview for CD45RA Antikörper (FITC) (ABIN2144603)

Target

Alle CD45RA Antikörper anzeigen
CD45RA

Reaktivität

  • 100
  • 9
  • 6
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 116
  • 1
  • 1
Maus

Klonalität

  • 117
  • 1
Monoklonal

Konjugat

  • 31
  • 16
  • 14
  • 7
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD45RA Antikörper ist konjugiert mit FITC

Applikation

  • 110
  • 28
  • 27
  • 25
  • 19
  • 18
  • 15
  • 14
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Klon

4AHI100
  • Produktmerkmale

    4AHI100 reacts with the 220 kDa isoform A of CD45. This is clustered as CD45RA, and is expressed on naive/resting T cells and on medullart thymocytes. In comparison, CD45RO is expressed on memory/activated T cells and cortical thymocytes. CD45RA and CD45RO are useful for discriminating between naive and memory T cells in the study of the immune system.

    Isotyp

    IgG2b
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Testdurchführung

    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C
  • Target

    CD45RA

    Andere Bezeichnung

    CD45RA
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